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. 2016 May 23;60(6):3709–3716. doi: 10.1128/AAC.00009-16

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Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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FIG 3 — mRNA expression levels of pmrA, phoP, pmrC, and crrC in A4528-derived strains. (A) The relative mRNA expression levels of pmrA and phoP in A4528 crrB(N141I) were determined and compared with that in the A4528 wild type. (B and C) mRNA expression levels of pmrA (B) and pmrC (C) in the A4528 wild-type strain and strains A4528 crrB(N141I), A4528 crrB(N141I) ΔpmrAB, A4528 crrB(N141I) ΔcrrC, A4528 crrB(N141I) ΔcrrC carrying a pACYC184 plasmid, and A4528 crrB(N141I) ΔcrrC carrying a pACYC184-3062 plasmid were determined and compared. (D) Relative fold expression of crrC in strains A4528 crrB(N141I), A4528 crrB(N141I) ΔpmrAB, and A4528 crrB(N141I) ΔcrrC compared with that in the A4528 wild-type strain. The mRNA expression of each strain was calculated by qRT-PCR independently in quadruplicate, and statistical analysis was performed using Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05; ND, not detected). The error bars represent SD.