Figure 5.

Quantification of IL10⁺F4/80⁺Ly6C⁺ myeloid cell populations in draining lymph nodes and spleen by flow cytometry. Cells were activated with PMA and ionomycin for 4 h in the presence of GolgiStop and GolgiPlug. After incubation, cells were harvested and stained on their surface with anti‐Ly6C and anti‐F4/80 monoclonal antibodies. For intracellular staining, cells were fixed, permeabilized, and stained with anti‐IL‐10 monoclonal antibody. (A) Representative plot and number of IL‐10⁺F4/80⁺Ly6C⁺ myeloid cell populations in dLNs at day 7 and (B) representative plot and number of IL‐10⁺F4/80⁺ Ly6C⁺ myeloid cell populations myeloid cells in spleen at day 7 of healthy, CIA, and eASC‐treated CIA mice. Data are represented by the mean and the standard error of the mean. **P < 0.01 and ***P < 0.001 represent the significance analyzed by the Mann–Whitney test (healthy, n = 5; CIA, n = 7; and eASC‐treated CIA, n = 15). Results represent four experiments.