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. 2016 Feb 26;16(6):1751–1765. doi: 10.1111/ajt.13690

Figure 6.

Figure 6

Influence of dexamethasone and azithromycin on IL‐1α induced IL‐8 and IL‐6 protein secretion by human lung fibroblasts in vitro. Primary lung fibroblasts (PLF) were treated with the indicated concentrations of dexamethasone (A, C, and E) or azithromycin (B, D, and F) for 1 or 24 h, respectively, after which IL‐1α (100 pg/mL) (n = 3) or media from primary lung epithelial cells (PLEC) challenged with live Pseudomonas aeruginosa strain 4616 (4616) (n = 6) was added and the cells were incubated for 24 h. To investigate the role of IL‐1α, the conditioned media from PLEC was pre‐incubated for 1 h with IL‐1α (4 μg/mL) neutralizing antibody. Secretion of IL‐6 (A and B) and IL‐8 (C–F) was quantified. Data were analyzed using paired t‐tests and presented as mean ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001.