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. 2016 May 25;6:26612. doi: 10.1038/srep26612

Figure 6. Expression of LactC2 fusion proteins in P. putida.

Figure 6

Cells of P. putida expressing sfGFP-LactC2 (A,B) or mTurquoise-LactC2 (C,D) were grown in mineral salts medium with sodium octanoate to promote PHA granule formation. Cells were stained with Nile red in (A,C) to visualize PHA granules. From left to right: bright field, red (top row) or green/turquoise (bottom row) channel and overlay images. Note, formation of globular inclusions visible in bright field that are stained by Nile-red (PHA granules) but that show no sfGFP-LactC2 or mTurquoise-LactC2 fluorescence. Scale bars correspond to 2 μm.