Skip to main content
. 2016 May 25;6:26655. doi: 10.1038/srep26655

Figure 1. Indirect Immuno-Fluorescence Assay [IFA] detection of TTSuV1 replication in human PBMCs.

Figure 1

Recombinant TTSuV1culture, rescued by transfection of human PBMCs with the circularized viral genome, was passaged three times in human PBMCs. A representative image from the third passage is depicted. (A)–Apple green, nuclear fluorescence in human PBMCs infected with TTSuV1 and stained with an anti-TTSuV1 ORF1- specific antibody, (B)–Blue, nuclear counter-staining with DAPI, (C)–Overlay image of A and B showing the nuclear localization of the replicating TTSuV1. Untransfected negative controls did not show specific fluorescence [image not shown].