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. 2016 May 25;6:26655. doi: 10.1038/srep26655

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Copyright © 2016, Macmillan Publishers Limited

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(A,B) ELISA for the detection of antibody responses to TTSuV1: Fig. 4A–Antibody responses to the TTSuV1 ORF2 protein in human sera (N = 40), Fig. 4B–Antibody responses to the TTSuV1 ORF2 protein in swine sera (N = 20). The mean optical density values of duplicate values are depicted. The cutoff value for the human sera (0.125) was calculated as the lowest quartile value of the data set +/− two standard deviations. The cutoff value for the swine samples (0.16) is the value of a negative control sample which was obtained after screening a panel of field swine sera.