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. 2016 May 25;6:26662. doi: 10.1038/srep26662

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Copyright © 2016, Macmillan Publishers Limited

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PBMCs were obtained from healthy rs9577873 genotyped controls (CC = 19, CT = 15–16) and were stimulated with LPS to investigate IL-1β, TNF-α and IL-6 production and with a cocktail of anti-CD3 and anti-CD28 antibodies to study IFN-γ, IL-17 and IL-10 production. An ELISA was used to detect IL-1β (a), IL-6 (b), TNF-α (c), IFN-γ (d), IL-17 (e) and IL-10 (f) in the cell culture supernatants. Data are shown as mean ± SD.