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. 2016 May 25;6:26583. doi: 10.1038/srep26583

Figure 4. Effect of endophytes (CATDLF5 and CATDLF6) on expression of genes involved in vindoline biosynthesis.

Figure 4

Transcript abundance of (a) STR, (b) SGD, (c) T16H, (d) 16OMT, (e) D4H, (f) DAT was analyzed. NC- the natural control plants that originated from C. roseus seeds that were not treated with any bacteriocide and fungicide and contained all the naturally occurring endophytes present in the plants. C- the endophyte-free control plants that originated from C. roseus seeds treated with bactericides and fungicides and were thus devoid of any naturally occurring endophyte. The endophyte-free control (C) was used as the calibrator. For normalization, C. roseus actin gene was used as the endogenous gene. Data are means ± SD (n = 3 biological replicates) and Y-axis represents relative quantity (RQ). Asterisks indicate significant differences as compared to the endophyte-free control (C) (Duncan’s multiple range test *P < 0.05).