Figure 3.
(A) Measured, fitted mitochondrial and fitted cytosolic carbon-nitrogen MMIDs of glutamate after 24h culture in labeled medium. Complete MMIDs are shown as array plots, selected isotopomers as bar charts. (B) Model of glutamate metabolism (simplified) with fitted MMIDs. Mitochondrial glutamate (upper) reflects synthesis from glutamine, while cytosolic glutamate (lower) is affected by aminotransferase activity, resulting in a smaller fraction 13C515N1. (C) Measured and fitted carbon-nitrogen MMIDs of proline, as in A.
