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. 2016 May 25;6:26557. doi: 10.1038/srep26557

Figure 3. FAM83H regulates the organization of the keratin cytoskeleton in HAM2 cells.

Figure 3

(a,h) HAM2 cells were transfected with FAM83H-FLAG or the control vector and analyzed by Western blotting (a), immunofluorescence (b–g), and a co-immunoprecipitation assay (h) using the indicated antibodies. In (b), insets indicate magnified images at the areas enclosed by white squares on a cell with (region 1) or without (region 2) the expression of FAM83H-FLAG. In (h), asterisks indicate non-specific bands because they were detected in the lanes of immunoprecipitates (IP) from not only FAM83H-FLAG-expressing cells, but also control cells. (i,j) HAM2 cells were transfected with FAM83H siRNA or control siRNA and analyzed by Western blotting (i) or immunofluorescence (j). In (j), the cell edge of a FAM83H-depleted cell is indicated by the dotted line. In (b–g,j), DNA was stained with DAPI (blue) and scale bars indicate 10 μm.