Figure 6. Treatment of HAM3 cells with D4476 disorganizes the keratin cytoskeleton and impairs the formation of desmosomes.

HAM3 cells were cultured in IMDM medium for 24 h and then treated with 100 μM D4476 or DMSO (control) for 1 h. Cells were analyzed by immunostaining with the indicated antibodies (a–e) or dispase-based dissociation assay (f). In (a–e), DNA was visualized by DAPI (blue), the edge of cell sheets was indicated by dotted lines (b,c), and scale bars indicate 20 μm. Ph, phase-contrast. In (f), cell sheets were rigorously agitated on a vortex mixer for the indicated times. Arrows indicate fragments of cell sheets.