Figure 5. CDR-H3 clusters associated with vaccination in non-carrier children.

(A) Six CDR-H3 clusters in order 5 were selected by both linear support vector classification and logistic regression. An open circle marks the clone with the highest PageRank score. The corresponding sequence, listed below, was connected to other similar clones with an indel-free Hamming distance of 1. (B) The six clusters were further extended with two rounds of Hamming distance 1 as described in the text. Percentages of the reads belonging to the new clusters rose significantly in post-vaccination samples only in the non-carriers. In family 1 and 4 there were two amplified clusters. There was only one new cluster in family 2 and 3. Vaccination was associated with at least a 0.5% increase in cluster reads for all non-carrier children.