FIGURE 5.

Quantification of ammonium uptake in yeast cells expressing glomeromycotan AMTs. Yeast cells expressing the stated genes from the plasmid pDR196sfi were grown over night in synthetic complete medium lacking uracil (HC-U), washed and cultured in liquid medium containing a starting concentration of 2 mM ammonium. Samples were taken after 10, 30, 60, 120, 180, 240, and 300 min, and residual ammonium was determined. Yeast cells expressing ScMEP1 and GintAMT1 took up ammonium quite rapidly. GintAMT2 and GintAMT3 expressing cells imported ammonium at a slower rate, but clearly above background level (“Vector”). Bars show average of 3–4 experiments and standard deviation.