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. 2016 May 25;7:679. doi: 10.3389/fpls.2016.00679

FIGURE 6.

FIGURE 6

Effect of N availability on GintAMT3 gene expression. (A) Real-time RT-PCR analysis of GintAMT3 mRNA levels in the ERM of R. irregularis developed in liquid M-C medium in the presence of either 3.2 mM (High N) or 0.8 mM nitrate (Low N) and exposed for different periods of time to a N-free medium. (B) Effect of N addition to the N-starved mycelium on GintAMT3 expression. Gene expression was analyzed by real-time RT-PCR in ERM grown in 25% N media, maintained for 48 h in a N-free media (Control) and exposed for 24 h to 3 mM (High) or 30 μM (Low) of NH4+ or NO3-, or 5 mM glutamine. Control plates were supplemented with H2O. Data were calibrated by the expression values obtained for the gene encoding the EF1α. Error bars represent SE of the mean of three independent experiments. ∗: statistically significant (p < 0.05) in comparison to the respective control value.