Figure 5.
In vitro transcription of Mtb iron regulated genes in the presence of IdeR. Transcript generated by Msm RNAP using a bfrB template (A) or a template of the iron and IdeR repressed gene mbtB (C). When indicated IdeR and Ni+2 were present in the reaction which was carried out as described in experimental procedures. Radiolabelled transcripts were separated by electrophoresis and detected by autoradiography. Shown are the results of one representative experiment that was repeated 4 times. Panels B and D show the densitometric quantification of the gels shown in A and C respectively.