Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 18;98(5):864–877. doi: 10.1111/mmi.13166

Figure 8.

Figure 8

An Lsr2 binding sequence located within the IdeR binding region is necessary for Lsr2-mediated repression of PbfrB. A. Schematic representation of the intact upstream sequence of bfrB harboring an Lsr2 binding sequence identified in protein binding microarrays (shown in bold), or a 5bp deletion (ΔTTATT) in the spacer region between IB2 and IB3 that destroys the Lsr2 binding site. lacZ fusions were generated with the intact and the deleted promoter and introduced into Msm WT and Δlsr2. B. β-galactosidase activity from the intact (filled bars) or deleted (open bars) promoter fusions in Msm (WT) and Δlsr2 strains grown under repressive (low iron) conditions. The deletion in the Lsr2 binding site resulted in (~4 fold) derepression of the promoter in the wild type Msm but did not lead to further derepression of the promoter in the Δlsr2 indicating that repression of PbfrB in low iron is partially dependent on an intact Lsr2 binding site present in the spacer sequence between IB2 and IB3. **, p<0.005, n.s. not significant.