Table 1.
Strains and Plasmids
| Strains | Specification | Source |
|---|---|---|
| Msm strains | ||
| SM3 | mc2155 ideR null mutant | (Dussurget et al., 1996) |
| SM197 | mc2155 lsr2 deletion mutant. | (Colangeli et al., 2007) |
| SM17 | mc2155 carrying pSM128 | (Rodriguez et al., 1998) |
| SM154 | mc2155 carrying pSM393 | (Gold et al., 2001) |
| SM160 | mc2155 harbouring pSM475 | This study |
| SM163 | SM3 harbouring pSM475 | This study |
| SM173 | mc2155 harbouring pSM519 | This study |
| SM176 | mc2155 harbouring pSM526 | This study |
| SM177 | mc2155 harbouring pSM527 | This study |
| SM178 | mc2155 harbouring pSM529 | This study |
| SM193 | mc2155 harbouring pSM641 | This study |
| SM224 | SM197 harbouring pSM128 | This study |
| SM225 | SM197 harbouring pSM475 | This study |
| SM228 | SM225 transformed with pSM895 | This study |
| SM230 | SM197 harbouring pSM641 | This study |
| SM232 | SM197 harbouring pSM393 | This study |
| SM234 | SM197 harbouring pSM526 | This study |
| SM244 | SM197 harbouring pSM519 | This study |
| SM245 | SM197 harbouring pSM527 | This study |
| SM246 | SM197 harbouring pSM529 | This study |
| Plasmids | ||
| pSM128 | Mycobacterial integrative vector containing a promoter-less lacZ | (Rodriguez et al., 1998) |
| pSM207 | PSL1180 containing a T4 and a SinR T2T3 terminator flanking a unique Pst1 site | This study |
| pSM393 | transcriptional fusion of PbfrA lacZ in pSM128 | This study |
| pSM475 | transcriptional fusion of PbfrB lacZ in pSM128 | This study |
| pSM519 | pSM475 with IB3 mutated | This study |
| pSM526 | pSM475 with IB1 and IB2 deleted | This study |
| pSM527 | pSM475 with 5 bp deletion in the sequence between IB2 and IB3 | This study |
| pSM529 | pSM475 with IB1 deleted | This study |
| pSM641 | transcriptional fusion of PmbtB lacZ in pSM128 | This study |
| pMV261 | expression vector | (Stover et al., 1991) |
| pSM895 | lsr2 cloned into pMV261. Δlsr2 complementing plasmid | This study |
| pSM669 | pET30 derivative-Lsr2 expression plasmid,. | (Colangeli et al., 2007) |
| pSM918 | pET28TEV derivative-IdeR expression plasmid | This study |
| pSM495 | 200 bp upstream of Mtb mbtB and 250 bp downstream of the start codon cloned into Zero blunt Topo (Invitrogen). | This study |
| pSM749 | pSM207 carrying 387 bp upstream and 65 bp of Mtb bfrB orf | This study |
| pSM750 | pSM207 carrying 387 bp upstream and 65 bp of Mtb bfrB orf with IB1 deleted. | This study |
| pARN104 | pUC18 containing the Mtb rRNA promoter PCl1 and 180 bp downstream DNA followed by a transcriptional terminator from Rv1324 | Tare et al., 2012 |