IHC analysis of F4/80 expression, a pan-macrophage marker, by macrophages
surrounding hydrogels explanted after 3 days (a–c), 10 days (d–f),
and 21 days (g–i). Hydrogels were crosslinked with glutaraldehyde at
concentrations of 0.05% (a, d, g), 0.1% (b, e, h), or 0.3% (c, f, i). (j)
Negative control (delete primary) and (k) positive controls (spleen). Scale bar
is 200μm. (l) Average staining intensity. Statistical significance was
determined using two way ANOVA and Tukey's post-hoc analysis.