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. Author manuscript; available in PMC: 2016 May 25.
Published in final edited form as: Mol Cell. 2011 Jun 24;42(6):719–730. doi: 10.1016/j.molcel.2011.04.025

Figure 5. Accumulation of Glycolytic Intermediates and NADPH in Cells Expressing Acetylation Mimetic K305Q Mutant.

Figure 5

(A) Cells expressing acetylation mimetic K305Q mutant of PKM2 accumulate higher levels of glycolytic intermediates. Metabolites in H1299 cells wild-type (H1299/PKM2) or K305Q mutant (H1299/PKM2K305Q) PKM2 were analyzed by LC-MSMS. The ratios of individual metabolites in H1299/PKM2 expressing over H1299/PKM2K305Q-expressing cells are shown from three independent samples. PKM2 knockdown efficiency and re-expression were determined by western blot. Error bars represent ±SD for triplicate experiments.

(B) H1299/PKM2K305Q cells accumulate NADPH. Cells used in the assay were the same as in (A). NADPH was measured using a NADPH kit according to the manufacturer’s protocol. Error bars represent ±SD for triplicate experiments.