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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Cardiovasc Electrophysiol. 2015 Oct 13;27(1):110–119. doi: 10.1111/jce.12833

Figure 5. Cx43 localization does not change with MS.

Figure 5

(A) An example of triton-X extraction products showing total (pre-extraction), soluble and insoluble fractions. Consistent extraction into the soluble fraction was achieved as demonstrated by the separation of the P1 and P2 bands generally found in the junction (insoluble fraction) from the P0 band generally not in the junction (soluble fraction). (B) Westerns for T-Cx43, exogenous Cx43 (HA-tag) and N-cadherin. (C) Summary of change in T-Cx43 expression (bottom left) and in exogenously expressed Cx43 (HA, bottom right). Cx43 expression was normalized to N-cadherin, and expressed as fold-change relative to baseline Cx43 expression. A value of 1 indicates equal Cx43 expression in insoluble fractions at baseline and MS. (AdGFP n=5 each; AdCx43 n=5 each, Ad368A n=6 each; Ad368E baseline n=4, MS n=5; p=NS for all groups).