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. Author manuscript; available in PMC: 2016 May 25.
Published in final edited form as: J Phys Chem B. 2016 Jan 22;120(4):715–723. doi: 10.1021/acs.jpcb.5b12339

Figure 3.

Figure 3

Long-term photo-CIDNP NMR data collection in the presence of FL and FMN. Cumulative signal-to-noise ratio of 200 µM Trp (1Hη2 resonance) in a 13C PRINT photo-CIDNP experiment (argon-ion laser in multiline mode, 0.5 Watt laser irradiation power, 0.1s irradiation time) upon long-term data collection. Either 60 µM total FL or 200 µM total FMN were used. All experiments included catalytic amounts of the GO and CAT enzymes (see Experimental Methods for details). The experiments with FMN also included catalytic amounts of the NR enzyme (0.3 units). All other sample conditions and data acquisition parameters were as in the legend of Figure 1. Seventy-five successive data sets, comprising 4 scans each, were collected and cumulative signal-to-noise was calculated according to eq. (2). Three-to-four experiments were performed for each data point. Uncertainties are expressed as ± one standard error.