Table 2.

Ethanol synthesis, biomass, yield and ATP of S. cerevisiae transformants with increased expression of fbp1 gene of E. coli and the wild-type strain strain on the fourth day of fermentation.

Strain	Ethanol, g L−1	Biomass, g L−1	Ethanol, g g−1 of biomass	ATP, µM g−1
ВY4742/pRS42H	21.3 ± 0.9	3.7 ± 0.2	5.7 ± 0.15	7.75 ± 0.18
BY4742/fbp1_7	23.3 ± 1.1	3.9 ± 0.4	6.0 ± 0.12	4.76 ± 0.10
BY4742/fbp1_13	24.5 ± 1.2	3.9 ± 0.3	6.2 ± 0.20	5.31 ± 0.12

Alcoholic fermentation was performed using a 10% glucose containing medium at 30°C under semi-anaerobic condition (120 revolutions/min) for 4 d as described elsewhere.⁸ The concentration of ethanol in fermentation media was determined using alcohol oxidase/peroxidase-based enzymatic kit “Alcotest.”¹⁹ ATP was measured by coupled enzymatic reactions with hexokinase and glucose-6-phosphate dehydrogenase as described elsewhere.²⁰