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. Author manuscript; available in PMC: 2016 May 25.
Published in final edited form as: Int J Cancer. 2014 Nov 11;136(10):2341–2351. doi: 10.1002/ijc.29301

Figure 4.

Figure 4

Silencing of p100 is associated with cleavage furrow defects and cytokinesis failure. (a) Table summarizing the number of abnormal anaphases and multipolar spindles observed in cells expressing p100- and Luc-shRNA. Centrosomes and mitosis were detected by costaining with anti-γ-tubulin and phospho-Aurora A. Anaphase bridges and multipolar spindles were quantified manually in 100 cells. (b) Index cases exemplifying: (b.1) anaphase bridge formation; (b.2) delay cleavage furrow and (b.3 and b.4) multipolar anaphases (*p < 0.01). (c–e): Representative picture of metaphase spreads of control or silenced p100 expressing OCI-LY3, RCK8 and Daudi cells. Full karyotype analysis is shown in Supporting Information Table 1C.