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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Plant J. 2016 Jan;85(2):320–333. doi: 10.1111/tpj.13099

Figure 6. Spatial determinants of IRT1-dependent root iron uptake.

Figure 6

(a) Phenotype of 16 day-old wild-type (WT), irt1-1 and irt1-1 transformed with IRT1-IRES∷IRT1-2xYpet-NLS under the control of the indicated SWELL promoters.

(b) Confocal microscopy image of the IRT1 SAND line

(c) Confocal microscopy image of the IRT1 RED TIDE line

(d) Immunofluorescence analysis on root cross-section from iron-deficient (-Fe) and iron-sufficient (+Fe) wild-type (WT) plants and irt1-1, using anti-IRT1 antibodies.

(e) Phenotype of 4 week-old wild-type (WT), irt1-1 and irt1-1/EXP7∷IRT1-IRES∷IRT1-2xYpet-NLS/SUC2∷IRT1 plants grown on soil.

Scale bars: 50 μm.