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. Author manuscript; available in PMC: 2016 Dec 17.
Published in final edited form as: Nature. 2015 Dec 9;528(7582):422–426. doi: 10.1038/nature16142

Figure 1. Inhibition of the BRCA1-PALB2 interaction in G1 is CRL3-KEAP1-dependent.

Figure 1

a, Micrographs of irradiated (2 Gy) G1-synchronized U2OS cells processed for γ-H2AX, BRCA1 and BRCA2 immunofluorescence. b, Quantitation of the experiment shown in a and Extended Data Fig. 1d. ASN, asynchronously dividing. WT, wild type (mean ± s.d., N=3). c, Immunoprecipitation (IP) of PALB2 from extracts prepared from mock- or X-irradiated 293T cells synchronized in S or G1 phases. A normal IgG IP was performed as control. Cyclin A staining ascertains cell cycle synchronization. For gel source data, see Supplementary Figure 1. d, Quantitation of the experiment shown in Extended Data Fig. 3a. 53BP1Δ U2OS cells transfected with the indicated GFP-PALB2 vectors and siRNAs were irradiated (20 Gy) before being processed for microscopy (mean ± s.d., N=3). e, Normal IgG and PALB2 IPs from extracts prepared from synchronized and irradiated 293T cells of the indicated genotypes.