Extended Data Figure 3.

a, Representative micrographs of the experiment shown in Fig. 1d. b, Schematic representation of human KEAP1 gene organization and targeting sites of sgRNAs used as described in Extended Data Fig.1. a. The indels introduced by CRISPR/Cas9 and their respective frequencies are indicated. c, Immunoprecipitation (IP) of PALB2 from extracts prepared from irradiated 293T cells. IP with normal IgG was performed as a control. d, 293T cells with the indicated genotypes were transfected with the indicated HA-KEAP1 constructs, synchronized in G1 or S phases and irradiated. Cells were processed for PALB2 immunoprecipitation (IP). EV, empty vector. e, Quantification of U2OS 256 cells transfected with the indicated GFP-PALB2 mutants and mCherry-LacR-BRCA1. Cells were also stained with a Cyclin A antibody to determine cell cycle position (N=3). f, Quantification of U2OS 256 cells transfected with GFP-PALB2 and mCherry-LacR-BRCA1-CC (WT or K1406R mutant). Cells were also stained with a Cyclin A antibody to determine cell cycle position. This panel shows that the sole lysine in the PALB2-interaction motif of BRCA1 is not involved in the cell cycle regulation of the PALB2-BRCA1 interaction.