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A high dose (1 μM) of the GPER agonist (G-1) inhibits ESR1 and ESR2 induced proliferation. MCF-7 cells were exposed to the ESR agonist (10 nM E2, A), the ESR1 specific agonist (100 nM PPT, B), or the ESR2 specific agonist (100 nM DPN, C), individually and in the presence of 1 μM G-1. Absorbance at 570 nm was measured and expressed as percent of control. Bars are Mean ± SEM of at least 1 experiment. Asterisk (*) indicates significant differences from control (P < .05).
