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. 2016 Feb 1;101(4):1615–1626. doi: 10.1210/jc.2015-3410

Figure 1.

Figure 1.

miR-155 is regulated by TNFα in human and 3T3–L1 adipocytes. A, The expression of miRNA in TNFα (5, 10, and 15 ng/mL for 24 h) incubated human adipocytes was quantified by microarrays and represented in a Table, as described in Materials and Methods. B, miR-155 expression in human adipocytes and the effect of 24 hours' incubation with TNFα was confirmed by qPCR. C, The effect of TNFα on miR-155 expression was studied in 3T3–L1 adipocytes. Cells were differentiated for 10 d and then incubated with different doses of TNFα (5, 10, 15, and 20 ng/mL) for 24 h or (D) exposed to 15 ng/mL of TNFα at different times (3, 6, 12, and 24 h). E, miR-155 was quantified in 3T3–L1 adipocyte supernatant under TNFα condition (15 ng/mL, 24 h). F, The expression of miR-155 was quantified by qPCR in epididymal adipose tissue of TNFα knockout mice (TNFα−/−). RNU6 or SNORD68 were used as the endogenous controls. The data are expressed as relative expression ratios of at least three independent experiments. The values are presented as means ± SEM. n.d., not detected. *, P < .05; **, P < .01; ***, P < .001.