Figure 1.

UFs having Med12 somatic mutations showed an up-regulation of β-catenin, Wnt4, and Wisp1 as compared with Med12-negative adjacent normal myometrium. A, Protein lysates were prepared from paired myometrium (M) and uterine fibroid (F; n = 5) from five individual subjects. Equal amounts of each protein lysates (30 μg) were analyzed by Western blots using anti-β-catenin, anti-Wnt4, and anti-Wisp1 antibodies. B, The intensity of each protein band was quantified using image-analyzing software, normalized to corresponding β-actin, and relative values were used to generate data graphs. Each underline shows UFs and the adjacent myometrium from the same patient. P1, P2, P3, P4, and P5 indicate fibroid subjects. Med12 mutation status in fibroids from subjects P1, P2, P3, P4, and P5 are 130G>A, 107T>C, 105A>T, 131 G>A, and 105A>T, respectively.