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. 2016 May 25;11(5):e0156079. doi: 10.1371/journal.pone.0156079

Fig 1. 10.1.1 Ab induces proliferation of LECs in vitro.

Fig 1

Pooled axillary, brachial, and inguinal LNs were enzymatically digested and plated into chamber slides. Cells were treated with antibody for 5 d and stained to identify proliferating LECs. A). Immunostaining of hamster IgG-treated cultures identifies Prox1+ LECs (red) and Ki67+ proliferating cells (green), and confirms nuclear location of Prox1 and Ki67 by blue nuclear DAPI staining (right panel). B). Immunostaining of 10.1.1 Ab-treated cultures identifies a number of Prox1+ and Ki67+ proliferating LECs (e.g. arrowheads, left panel), and confirms nuclear location of Prox1 and Ki67 by DAPI staining (arrowheads, right panel). C). The Ki67+Prox1+ or Ki67+Prox1- cells from 6 preparations were counted in five fields for each sample, and the percentage of each population was determined. 10.1.1 Ab-treated samples display increased proliferating LECs compared to control Hamster IgG-treated samples. Significance was determined using a Wilcoxon Ranked Sum test for paired samples. *: p<0.05. Standard errors are indicated.