Fig 2. 10.1.1 Ab or lymphocyte co-culture similarly block in vitro tube formation.

SV-LECs were treated overnight with 30 μg/ml Hamster IgG or 10.1.1 Ab or co-cultured overnight with lymphocytes. Cells were trypsinized and plated on growth factor-reduced Matrigel at equal densities and allowed to form tubes. A). Cells were stained with Calcein AM and 4x images were analyzed. Scale bars are indicated. B). The percent area occupied by the tubes was quantified using Image J. 10.1.1 Ab treatment and lymphocyte co-culture similarly reduced tube formation potential of SV-LECs while Hamster IgG control Ab did not impact tube formation. Significance was determined using a Mann Whitney U test for unpaired samples. n = 5, * p<0.05.