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. 2016 May 25;11(5):e0156079. doi: 10.1371/journal.pone.0156079

Fig 4. 10.1.1 Ab induces coordinate accumulation of lymphatic endothelial and stromal cells.

Fig 4

Pooled LNs were enzymatically digested and stained with CD45, CD31, and Podoplanin antibodies for flow cytometry analysis. Viable cells were gated as CD45- to detect all stroma and then further gated to identify LEC (CD31+ Podoplanin+), FRC (CD31- Podoplanin+), BEC (CD31+ Podoplanin-), and DN stromal cells (CD31- Podoplanin-). A). 10.1.1 Ab-injected mice display an increased percentage of increased CD45- cells. B). Populations are shown as a percent of CD45- cells. All cell types remain proportional in the 10.1.1 Ab-injected mice indicating a coordinate expansion of LN stromal cell populations in response to 10.1.1 Ab treatment. Significance was determined using a Mann Whitney U test for unpaired samples. n = 6, p<0.05. Standard errors are indicated.