Skip to main content
. 2016 May 25;11(5):e0156079. doi: 10.1371/journal.pone.0156079

Fig 7. Lymph node lymphangiogenesis involves LEC and non-LEC proliferation.

Fig 7

A). Schematic of the experimental timeline for the BrdU pulse-chase experiment. Mice were injected i.p. 10.1.1 Ab or control Hamster IgG at 0 h. At 16 h, mice were injected i.p. with BrdU. Two h after BrdU administration, mice were either sacrificed as part of the pulse cohort or injected i.p. with thymidine to stop further BrdU incorporation. The pulse-chase cohort was sacrificed at 38 h after Ab injection. B-E). Popliteal LN sections were stained with anti-LYVE-1 (red) and anti-BrdU (green) antibodies. White boxes on low magnification images (left panels) identify the medullary sinus regions shown at higher magnification in the right panels. LNs from Hamster IgG-injected mice from pulse (B) and pulse-chase (C) cohorts display similar LYVE-1 staining patterns and no BrdU labeling of LYVE-1+ cells. BrdU-positive LYVE-1+ LECs are identified in the pulse-labeled (D, arrowheads) and pulse chase-labeled LNs (E, arrowheads) from 10.1.1 Ab-injected mice. Most of the BrdU-positive LYVE-1- non-LEC remain clustered near the growing edge of the sinus in the pulse (D, arrows) and pulse-chase cohorts (E, arrows) indicating that these proliferating cells do not contribute directly to the sinus growth. Scale bars are indicated.