Fig 5. γ-PGA and glutamate content of WT B. subtilis Bs916 and the ΔgltB mutant in the process of biofilm formation.

For glutamate detection, in addition to standard L-glutamate and double dilution to B. subtilis Bs916, all treatments were diluted tenfold for detection. For γ-PGA detection, all treatments were also diluted tenfold for detection.