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A final concentration of 10 g/L, 20 g/L, and 30 g/Lγ-PGA solution were added into the ΔgltB mutant in 4mL EM medium. The final concentration of 10 g/L and 20 g/Lγ-PGA were able to recover biofilm formation in the ΔgltB mutant, however, high final concentration of 30 g/Lγ-PGA inhibited biofilm formation in WT B. subtilis Bs916 and the ΔgltB mutant.
