Table 3. Identification of differentially expressed proteins in A549 cells infected with H7N9 and H1N1pdm09 influenza virus at 72hpi.
| Spots No. | Names | Accession | Score | MW(kDa)/PI | AV.ratio (H7N9/H1N1) | P value(T-test) |
|---|---|---|---|---|---|---|
| 1 | Heat shock 70 kDa protein 1A/1B | HSP71_HUMAN | 277 | 70294/5.4 | 2.45 | 2.00E-05 |
| 2 | Heterogeneous nuclear ribonucleoprotein D-like | HNRDL_HUMAN | 56 | 46580/10 | -2.57 | 3.60E-05 |
| *3 | Platelet-activating factor acetylhydrolase Ⅰb subunit beta | PA1B2_HUMAN | 138 | 25724/5.5 | -15.52 | 9.80E-05 |
| 4 | Prelamin-A/C | LMNA_HUMAN | 150 | 74380/6.6 | 2.26 | 0.00014 |
| *5 | F-actin-capping protein subunit alpha-1 | CAZA1_HUMAN | 192 | 33073/5.4 | -4.27 | 0.00027 |
| 6 | Heterogeneous nuclear ribonucleoprotein H | HNRH1_HUMAN | 392 | 49484/5.9 | 1.66 | 0.00028 |
| *7 | Ornithine aminotransferase | OAT_HUMAN | 38 | 48846/6.6 | -2.95 | 0.00029 |
| 8 | Peptidyl-prolyl cis-trans isomerase B | PPIB_HUMAN | 50 | 23785/9.9 | -8.39 | 0.00065 |
| 9 | Cofilin-1 | COF1_HUMAN | 213 | 18719/9.1 | -1.6 | 0.0008 |
| 10 | Transgelin-2 | TAGL2_HUMAN | 46 | 22548/9.3 | 1.97 | 0.0018 |
| 11 | Transcription elongation factor B polypeptide 1 | ELOC_HUMAN | 71 | 12636/4.6 | -3.53 | 0.0027 |
| 12 | Ezrin | EZRI_HUMAN | 107 | 69484/5.9 | 1.59 | 0.0028 |
| 13 | Macrophage-capping protein | CAPG_HUMAN | 155 | 38760/5.8 | 1.53 | 0.0031 |
| 14 | Proteasome subunit alpha type-1 | PSA1_HUMAN | 230 | 29822/6.2 | 1.52 | 0.0044 |
| 15 | Thioredoxin domain-containing protein 5 | TXND5_HUMAN | 38 | 48283/5.6 | -2.25 | 0.0058 |
| 16 | U6 snRNA-associated Sm-like protein LSm2 | LSM2_HUMAN | 119 | 10942/6.1 | 1.93 | 0.0058 |
| *17 | Eukaryotic translation initiation factor 5A-1 | IF5A1_HUMAN | 112 | 17049/4.9 | -5.96 | 0.0072 |
| *18 | Poly(rC)-binding protein 1 | PCBP1_HUMAN | 72 | 37987/6.8 | 1.72 | 0.0078 |
| 19 | Nucleophosmin | NPM_HUMAN | 208 | 32726/4.5 | -3.33 | 0.0083 |
| 20 | ATP synthase subunit alpha | ATPA_HUMAN | 122 | 59828/9.6 | -2.61 | 0.0087 |
| 21 | Aconitate hydratase, mitochondrial | ACON_HUMAN | 333 | 86113/7.9 | -3.57 | 0.0088 |
| 22 | Alpha-enolase | ENOA_HUMAN | 160 | 47481/7.7 | 1.97 | 0.009 |
| 23 | Myosin light polypeptide 6 | MYL6_HUMAN | 120 | 17090/4.4 | -3.29 | 0.0092 |
| 24 | Gelsolin | GELS_HUMAN | 164 | 86043/5.9 | 1.6 | 0.013 |
| 25 | Flavin reductase (NADPH) | BLVRB_HUMAN | 41 | 22219/7.9 | 1.5 | 0.014 |
| 26 | Phosphatidylethanolamine-binding protein 1 | PEBP1_HUMAN | 209 | 21158/7.8 | 1.58 | 0.016 |
| 27 | Actin, aortic smooth muscle | ACTA_HUMAN | 51 | 42381/5.1 | 2.25 | 0.019 |
| 28 | Superoxide dismutase [Mn] | SODM_HUMAN | 119 | 24878/9.1 | 4.23 | 0.019 |
| 29 | Microfibrillar-associated protein 1 | MFAP1_HUMAN | 65 | 51927/4.8 | -2.19 | 0.021 |
| 30 | Protein NDRG1 | NDRG1_HUMAN | 172 | 43264/5.4 | 1.69 | 0.021 |
| 31 | TAR DNA-binding protein 43 | TADBP_HUMAN | 56 | 45053/5.8 | 1.61 | 0.023 |
| 32 | Retinal dehydrogenase 1 | AL1A1_HUMAN | 95 | 55454/6.3 | -8.89 | 0.029 |
| 33 | Vasodilator-stimulated phosphoprotein | VASP_HUMAN | 114 | 39976/9.7 | 1.68 | 0.036 |
* Indicating that the differential proteins were validated by western blot analysis.