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. 2016 May 25;11(5):e0155832. doi: 10.1371/journal.pone.0155832

Fig 5. Vwf gene is cis-regulated in the brain and haplotype mapping revealed a minimal haplotype region.

Fig 5

(A) Cis-regulated expression QTLs within Hse6 from naïve tissues of RILs. Abbreviations: adr—adrenal gland; kdn—kidney; lv–left ventricle; skm—skeletal muscle. (B) Vwf is significantly cis-regulated in the brain eQTL, LRS >40. Upper red horizontal line: significant LRS genome-wide threshold at P ≤ 0.05. Lower blue horizontal line: suggestive LRS genome-wide threshold at P ≤ 0.63. (C) SNP haplotype map of the tested HSE susceptible and resistant inbred strains and additional not tested sub-colonies of some strains (PVG/seac; BN/NHsdMcw; SHR/NHsd; SHR/Ncrl; DA/Slc; DA/OlaHsd). Within the Hse6 region, 77 SNPs were found in RGD. Here we present the map with only 42 SNPs (between 160–165 Mb), in which the arrows point towards the only SNP marker position that showed the same allele in all resistant strains (blue allele) and a different allele in all susceptible strains (red allele). The flanking markers define a minimal haplotype region regulating HSE that is spanning over 161,438,840–161,843,906 bp on chromosome 4 indicating the influence of Vwf gene.