Fig 2. A specific set of miRNAs enhances the hypoxic response.

miRNAs were overexpressed using a btl-Gal4 driver in embryos exposed to 11% O₂. (A) No induction of the reporter was observed in embryos with the btl-Gal4 driver alone (negative control) and (B) in embryos overexpressing most of the microRNAs of the collection (miR-970 is shown as an example). (C) Expression of miR-190, (D) miR-274, (E) miR-280 or (F) miR-985 induced the Sima-dependent HRE-LacZ reporter. (G) Quantification of embryos in which the HRE-LacZ reporter was induced. Error bars represent SD; n ≥ 25 per group in three independent experiments.