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. 2016 May 25;11(5):e0156303. doi: 10.1371/journal.pone.0156303

Fig 4. Knockdown of S1PR2 inhibited bone resorption in BM cells induced by RANKL.

Fig 4

BM cells were uninfected, infected with a S1PR2 shRNA lentivirus, or infected with a control shRNA lentivirus (moi 20), and co-cultured with M-CSF and RANKL, with or without A. actinomycetemcomitans-stimulated media (Aa-media) treatment as described in Methods. Control groups of cells were cultured only with M-CSF with or without Aa-media treatment. (A) Representative images show bone resorption pits. Pictures were taken at 100x magnification. (B) Total areas of bone resorption pits /image were quantified. The data are representatives from three separate experiments (n = 3, *P<0.05, ** P<0.01, *** P<0.001).