Figure 2. The strain-specific anxiety- and despair-like behavioral responses to daily gavage are differentially affected by oral antibiotic treatment.
(A) Experimental timeline: vehicle or antibiotic mix were administered daily by gastric gavage (g.g.) for 14 days. Behavioral testing was performed before (baseline) and after treatment. Figure shows the results for the Elevated Plus Maze (EPM) and Forced Swim Test (FST) for NOD (B, C) and C57BL/6 (D, E) mice after oral treatment (g.g.). Baseline measurements for FST were performed in a separate cohort of mice (n=10) to avoid carryover effects (3 independent experiments with 8 mice per group/experiment for a total of n=24 mice per condition). Data are mean ± S.E.M; *p<0.05, **p<0.01, ***p<0.001 based on one-way ANOVA followed by Bonferroni’s post hoc test; n.s. indicates not significant.
Figure 2—figure supplement 1. Anxiety and despair-like behaviors after subcutaneous (s.c.) vehicle or antibiotic treatment.
(A) Experimental timeline: vehicle or antibiotic mix were administered daily (s.c.) for 14 days. Behavioral testing was performed before (baseline) and after treatment. Figure shows the results for Elevated plus maze (EPM) and Forced Swim Test (FST) for NOD (B–C) and C57BL/6 (D–E) mice after s.c. treatment. Baseline measurements for FST were performed in a separate cohort of mice (n=10) to avoid carryover effects of the FST (2 independent experiments with 10 mice per group/experiment for a total of n=20 mice per condition). Data are mean ± S.E.M; *p<0.05, ***p<0.001 based on one-way ANOVA followed by Bonferroni’s post hoc test; n.s. indicates not significant.