Figure 8. Cresol treatment decreases myelin gene expression.
(A) Transcript levels of oligodendrocyte lineage (Olig2), progenitor (Pdgfrα, Cspg4) and differentiation (Mag, Mog, Mbp, Cnp, Sox10) markers in oligodendrocyte progenitors cultured in differentiating conditions and treated with increasing concentrations of cresol (0, 10, 50 μM). DMSO was used as vehicle and negative control. Values were normalized to 36b4 mRNA levels and are referred as fold change relative to the control group (n=3 independent primary cultures). (B,C) Representative confocal images and quantification of early differentiated oligodendrocytes (CNP+/OLIG2+) after treatment with increasing concentrations of cresol (0, 10, 50 μM) for 24 hr. (D,E) Representative confocal images and quantification of oligodendrocytes (OLIG2+/DAPI+) treated with increasing concentrations of cresol (0, 10, 50 μM) for 24 hr. Scale bars, 20 μm; 10–15 fields (20×) per condition/experiment; n=2 independent primary cultures. Data are mean ± S.E.M; *p<0.05, ***p<0.001 based on one-way ANOVA with Dunnett's Multiple Comparison Test; n.s. indicates not significant