Figure 2. Energetic modes of C. elegans reproductive and dauer larvae.

(A) Respiration rates in terms of OCR difference between water-treated and azide-treated worms (n = 4 for each group). ANOVA shows that in both strains, L3 larvae consume significantly more oxygen than dauer larvae (F_1,12 = 1469, p < 0.001). There is also a minor effect of strain on oxygen consumption (F_1,12 = 6.864, p = 0.022), however there is no interaction between the larval stage and the strain (F_1,12 = 0.166, p = 0.691). Error bars show 95% confidence intervals. (B) Steady-state trehalose levels of daf-2 and daf-2;icl-1, L3 and dauer larvae (n = 3 for each group). L3 larvae produce less trehalose than dauer larvae (F_1,8 = 92.814, p < 0.001) independent of the strain (F_1,8 = 0.083, p = 0.781). Error bars show standard error of the mean. *p < 0.001.

DOI: http://dx.doi.org/10.7554/eLife.13614.005

Figure 2—figure supplement 1. Details of oxygen consumption rate measurements.

Oxygen consumption rates of L3 (top panels) and dauer (bottom panels) larvae of daf-2 (left panels) and daf-2;icl-1 (right panels). Dashed and solid lines indicate OCR after water (control) and 20 mM sodium azide injection, respectively.

DOI: http://dx.doi.org/10.7554/eLife.13614.006