(A) Respiration rates in terms of OCR difference between water-treated and azide-treated worms (n = 4 for each group). ANOVA shows that in both strains, L3 larvae consume significantly more oxygen than dauer larvae (F1,12 = 1469, p < 0.001). There is also a minor effect of strain on oxygen consumption (F1,12 = 6.864, p = 0.022), however there is no interaction between the larval stage and the strain (F1,12 = 0.166, p = 0.691). Error bars show 95% confidence intervals. (B) Steady-state trehalose levels of daf-2 and daf-2;icl-1, L3 and dauer larvae (n = 3 for each group). L3 larvae produce less trehalose than dauer larvae (F1,8 = 92.814, p < 0.001) independent of the strain (F1,8 = 0.083, p = 0.781). Error bars show standard error of the mean. *p < 0.001.
DOI:
http://dx.doi.org/10.7554/eLife.13614.005