Figure 6. Growth of GS-deficient yeast cells in media with different carbon sources.
(A) Growth of wild-type S. cerevisiae in YP + Glc medium in batch culture. The time of diauxic shift is shown with a dashed line. Error bars show standard deviation (n = 3). (B–D) Growth of wild-type (WT) or GS-deficient yeast in amino acid rich (YP) or minimal medium with glucose (B), ethanol (C) and acetate (D) as the carbon source. Note that particularly with acetate as the primary carbon source, GS mutants (∆icl1, ∆mls1, ∆dal7, ∆mls1/∆dal7 and ∆icl1/∆mls1/∆dal7) grow poorly regardless of amino acid availability.