(A, B) Steady-state trehalose levels of WT, ∆icl1 and ∆icl-1/∆mls1/∆dal7 strains in YP + Glc (A) and YP + Ac (B) media after 20 hr (post-diauxic shift, dark bars) and 48 hr (stationary phase, light bars). (C, D) Steady-state glycogen levels under the same conditions. n.d.: Not detected/below assay sensitivity range. (E, F) Desiccation tolerance of the indicated WT and mutant yeast cells, measured after 24 hr (E) or 30 days (F) of desiccation. Error bars show 95% confidence intervals. ***p < 0.001.
DOI:
http://dx.doi.org/10.7554/eLife.13614.015