Figure 7. Trehalose/glycogen synthesis and desiccation tolerance in GS-deficient yeast cells.
(A, B) Steady-state trehalose levels of WT, ∆icl1 and ∆icl-1/∆mls1/∆dal7 strains in YP + Glc (A) and YP + Ac (B) media after 20 hr (post-diauxic shift, dark bars) and 48 hr (stationary phase, light bars). (C, D) Steady-state glycogen levels under the same conditions. n.d.: Not detected/below assay sensitivity range. (E, F) Desiccation tolerance of the indicated WT and mutant yeast cells, measured after 24 hr (E) or 30 days (F) of desiccation. Error bars show 95% confidence intervals. ***p < 0.001.
Figure 7—figure supplement 1. Cell density, trehalose and glycogen levels in WT and mutant yeast strains.
(A) Maximum cell density attained by wild type and GS-deficient cells after 48 hr of growth in YP + Glc medium or YP + Ac medium. (B) Trehalose levels in WT, ∆hsp104 and ∆tps1 cells after 48 hr of growth in YP + Glc medium. (C) Glycogen levels in WT, ∆hsp104 and ∆tps1 cells after 48 hr of growth in YP + Glc medium. Error bars represent 95% confidence intervals. ***p < 0.001