Table 1.
FRET vs. EM model distances in the λ excisive recombination complex. R(FRET) values were determined by Seah et al. (2014) using in-gel fluorescence measurements for dye pairs whose positions were designed in the absence of a model for the λ excision complex architecture. Dyes positioned at sites 4,5,6,7,11, and 12 are predicted to be largely free from steric interference based on the EM structure. The FRET pairs involving these positions are in the first group below. Dyes positioned at site 3 (second group) and site 8 (third group) are predicted to have steric conflicts between the dye and the excision complex. Four additional sites (1,2,9, & 10) used in the study are on extended P and P' arms not present in the construct used for EM studies. See Seah et al. (2014) for descriptions of the site position nomenclature.
| Position i | Position j | R (FRET) (Å) | R (FRET) Model) (Å) | R (EM Model) (Å) |
|---|---|---|---|---|
| 7 [P+50T] | 6 [P+17B] | 56 | 57 | 50.0 |
| 4 [P-58B] | 5 [P-15T] | 70 | 72 | 70.6 |
| 5 [P-15T] | 12 [B+17B] | 76 | 77 | 78.8 |
| 4 [P-58B] | 6 [P+17B] | 76 | 70 | 67.9 |
| 5 [P-15T] | 6 [P+17B] | 89 | 80 | 81.1 |
| 6 [P+17B] | 11 [B-15T] | 93 | 81 | 85.3 |
| 7 [P+50T] | 4 [P-58B] | 99 | 125 | 108.9 |
| 11 [B-15T] | 12 [B+17B] | 103 | 86 | 82.5 |
| 3 [P-118T] | 12 [B+17B] | 80 | 69 | 64.1 |
| 3 [P-118T] | 5 [P-15T] | 82 | 77 | 95.3 |
| 3 [P-118T] | 11 [B-15T] | 99 | 108 | 100.0 |
| 8 [P+79B] | 3 [P-118T] | 56 | 56 | 41.4 |
| 8 [P+79B] | 11 [B-15T] | 74 | 65 | 75.2 |
| 8 [P+79B] | 5 [P-15T] | 96 | 109 | 81.3 |
| 8 [P+79B] | 6 [P+17B] | 96 | 107 | 85.4 |
| 8 [P+79B] | 4 [P-58B] | 108 | 158 | 124.8 |
| 8 [P+79B] | 12 [B+17B] | 116 | 73 | 68.2 |