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. 2016 May 25;5:e13881. doi: 10.7554/eLife.13881

Figure 4. Genetic interactions between Syntaxin 4 and Synaptotagmin 4.

Figure 4.

(A–E) Representative images of NMJs stained with antibodies to the postsynaptic marker Dlg (green) and the neuronal membrane marker HRP (magenta) to highlight the number of synaptic boutons; images are shown from Syx473/+ (A), Syt4BA1/+ (B), Syx473/+ Syt4BA1/+ (C), Syx473/+ Syt4BA1 (D), and Syx473 Syt4BA1/+ (E) animals. (F) Quantification of bouton number, normalized to controls. Blue line indicates the control mean. Data are presented as mean ± SEM. L = lethal. Scale bars = 20 μm (A–E). Statistical comparisons are fully described in Figure 4—source data 1, and are indicated here as ***p<0.001, **p<0.01, *p<0.05, ns = not significant; comparisons are with control unless indicated.

DOI: http://dx.doi.org/10.7554/eLife.13881.011

Figure 4—source data 1. Statistical data for Figure 4.
Sample size (n), mean, SEM, and pairwise statistical comparisons are presented for the data in Figure 4F.
DOI: 10.7554/eLife.13881.012