Fig. 5.

Effects induced by KCC2 and carbonic anhydrase inhibitors on the pharmacologically isolated GABAergic events. Effects induced by VU0240551 (10 μM) (a), CLP275 (100 μM) (b), or acetazolamide (10 μM) (c) on the slow synchronous field events occurring in the CA3 subfield during concomitant application of 4AP+CPP+CNQX (10 μM). In both panels, the initial control activity under 4AP treatment is also shown. d Enlarged portion of slow synchronous field events shown in panels a–c. e Bar graphs summarizing the change in interval of occurrence, duration, and amplitude of the slow synchronous events under control conditions (i.e., 4AP+CPP+ CNQX) and during further application of VU0240551 (10 μM), bumetanide (50 μM), CLP275 (100 μM), or acetazolamide (10 μM). Note that inhibiting either KCC2 or carbonic anhydrase activity ecreases the rate of occurrence, duration, and amplitude of the slow events while enhancing KCC2 activity increases the rate of occurrence and duration of these events. *p<0.001