Table 2. Nicotine metabolite levels in urine (ng/ mg creatinine) over 7 weeks after cessation of alcohol.

Analyte	Baseline (Week 1)	Week 4	Week 7	F, p-value
Cotinine	1467 ± 206	2529 ± 316*	2439 ± 325*	6.66, p=0.003
Cotinine glucuronide	2048 ± 391	3214 ± 565	3172 ± 333*	4.07, p=0.03
Total cotinine	3515 ± 555	5743 ± 786*	5611 ± 517**	7.64, p=0.004
3HC	9919 ± 1272	8678 ± 1231**	6454 ± 836**	9.82, p<0.001
3HC glucuronide	1669 ± 389	2048 ± 778	1282 ± 218	NS
Total 3HC	11589 ± 1594	10726 ± 1768	7736 ± 988**	5.83, p=0.01
Nicotine	582 ± 159	1224 ± 264	2023 ± 550**	6.13, p=0.009
Nicotine glucuronide	464 ± 93	914 ± 143*	983 ± 163**	7.33, p=0.002
Total Nicotine	1046 ± 223	2138 ± 341*	3006 ± 591***	9.98, p<0.001
Nicotine N'Oxide	621 ± 164	803 ± 146	1155 ± 246*	5.14, p=0.01
Cotinine N'Oxide	786 ± 90	794 ± 83	765 ± 78	NS
Nornicotine	102 ± 17	152 ± 26	143 ± 24	NS
Norcotinine	439 ± 60	447 ± 64	374 ± 51	NS

Nicotine metabolites measured in the subsample (N=22). Blood and urine samples were obtained before the first cigarette of the day at baseline (week 1), week 4 and week 7 after abstinence from alcohol. Shown are the mean metabolite levels in urine (ng/ mg creatinine ± SEM) and results from RM-ANOVAs across the three sessions. Significant differences from baseline are indicated as:

^*= p<0.05;

^**= p<0.01;

^***p<0.001.

3HC = trans-3′-hydroxycotinine; NS = nonsignificant.