Figure 4. Absence of mitochondrial ROS promotes NF-κB activation.

Representative western blot and densitometry quantification to detect expression of (A) phospho IKKα/IKKβ levels in sorted CD11c⁺ cells, (B) IκBα in cytoplasmic extracts and c-rel, p65 subunit (RelA) of NF-κB in nuclear extracts prepared from lung CD11c⁺ and CD11c- cells from PPARγ^ΔAPC and WT mice 1 hr after the last exposure to OVA, (C) IκBα in lung CD11c⁺ cells sorted from PPARγ^ΔAPC and WT mice 1 hr after the last exposure to Derp1 and (D) phospho IκBα (pIκBa) and total IκBα levels in whole cell extract and p65 levels in nuclear extracts from lung CD11c⁺ cells (sorted from tolerized mice), cultured for 1 hr in the presence or absence of DMNQ (200 µM). For all western blot analyses, expression of β-actin and LSD1 is shown as loading controls for cytoplasmic and nuclear extracts respectively and densitometric quantification is combined data from two independent experiments represented as mean ± SD. ND: not detected, *p< 0.05, **p< 0.01. See also Figure S5.