Figure 8.
(A) Scatterplot of percent area myotubes against the orientation of myotubes for each pattern shows a trend of increasing deviation from alignment with the patterned lines as percent area of myotubes increases. There is a tradeoff for increasing the amount of myotube formation and maintaining uniaxial organization of myotubes. (B) Scatterplot with human myotube orientation and percent area shows there is not a decrease in alignment with LAM lines (90°) with an increase in percent area myotubes and decrease in line spacing, as is observed with C2C12 myotubes. Error bars represent standard deviation.